PRO-E0233
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More information| Catalogue No. | PRO-E0233 |
|---|---|
| EC | 3.1.1.11 |
| Synonyms | Pectinesterase; pectin demethoxylase; pectin methoxylase; pectase; pectin methyl esterase; pectinoesterase; pectin pectylhydrolase |
| Nomenclature | CAZy [CE8 | carbohydrate esterase family 8] |
| Specific Activity | 27.48 U/mg |
| Accession No. | Q829N4 | NP_827552 | BAC74087.1 |
| Molecular Weight | 39613.8 Da |
| Biological Function | Catalyses the reaction: pectin + n H(2)O = n methanol + pectate |
| Comments | In a separate assay | the linear release of pectate from 89 % methyl-esterified pectin as detected simultaneously at 235 nm in 50 mM Tris/HCl buffer | pH 8.8 | by a large excess of pectate lyase (PRO-E0250) would suggest that this enzyme is acting in a processive fashion (unpublished data | Prozomix). The pH optimum is approx. 8.5 | where activity at 30oC rises from 35.72 U/mL at pH 7.5 (27.48 U/mg) to 51.1 U/mL (39.31 U/mg) |
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